nikon-structured illumination microscopy (3d n-sim) Search Results


3d n sim microscope  (Nikon)
99
Nikon 3d n sim microscope
3d N Sim Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nikon-structured+illumination+microscopy+(3d+n-sim)/Objectives/bio_rxiv__2020__06__17__156299-402-7-11
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n sim structured illumination super resolution microscope  (Nikon)
96
Nikon n sim structured illumination super resolution microscope
N Sim Structured Illumination Super Resolution Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nikon-structured+illumination+microscopy+(3d+n-sim)/N-SIM+S/pmc06992383-340-11-10
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nikon n sim microscope  (Nikon)
96
Nikon nikon n sim microscope
Nikon N Sim Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nikon-structured+illumination+microscopy+(3d+n-sim)/Illumination+Systems/pmc05708734-114-37-37
Average 96 stars, based on 1 article reviews
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n sim structured illumination nikon microscope  (Nikon)
99
Nikon n sim structured illumination nikon microscope
a Representative <t>SIM</t> micrograph of 4 h HIV-1ΔEnvNL4-3-GFP-Vpr <t>(VSV-G)-infected</t> <t>U2OS</t> cells showing SUGT1 and α-tubulin expression (aI). aII is a magnification of the dashed region in aI. aII1–4 are the magnifications of the dashed regions in aII. Fluorescence overlap spectrums of aII1–4 cropped regions, are shown. b HIV-1 IN and SUGT1 expression levels by WB in control and SUGT1-depleted U2OS cells after 48 h siRNA transfection and expression of exogenous HIV-1 IN for 24 h. Representative confocal micrographs of HIV-1 HA-IN expression in control and SUGT1-depleted U2OS cells (c) and percentages of cells showing nuclear or diffused HIV-1 IN (d). e Immunoprecipitation of HA-IN in control and HA-IN-overexpressing HEK293T cells and expression of indicated proteins by WB. WB and images are representative of three independent experiments. Means ± SEM are indicated from at least three independent experiments. P values were calculated using the two-way ANOVA test (****p < 0.0001).
N Sim Structured Illumination Nikon Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nikon-structured+illumination+microscopy+(3d+n-sim)/NIS-Elements/pmc07853140-119-12-15
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n sim e  (Nikon)
96
Nikon n sim e
a Representative <t>SIM</t> micrograph of 4 h HIV-1ΔEnvNL4-3-GFP-Vpr <t>(VSV-G)-infected</t> <t>U2OS</t> cells showing SUGT1 and α-tubulin expression (aI). aII is a magnification of the dashed region in aI. aII1–4 are the magnifications of the dashed regions in aII. Fluorescence overlap spectrums of aII1–4 cropped regions, are shown. b HIV-1 IN and SUGT1 expression levels by WB in control and SUGT1-depleted U2OS cells after 48 h siRNA transfection and expression of exogenous HIV-1 IN for 24 h. Representative confocal micrographs of HIV-1 HA-IN expression in control and SUGT1-depleted U2OS cells (c) and percentages of cells showing nuclear or diffused HIV-1 IN (d). e Immunoprecipitation of HA-IN in control and HA-IN-overexpressing HEK293T cells and expression of indicated proteins by WB. WB and images are representative of three independent experiments. Means ± SEM are indicated from at least three independent experiments. P values were calculated using the two-way ANOVA test (****p < 0.0001).
N Sim E, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nikon-structured+illumination+microscopy+(3d+n-sim)/N-SIM+E/pm36284196-274-14-16
Average 96 stars, based on 1 article reviews
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n sim module  (Nikon)
95
Nikon n sim module
a Representative <t>SIM</t> micrograph of 4 h HIV-1ΔEnvNL4-3-GFP-Vpr <t>(VSV-G)-infected</t> <t>U2OS</t> cells showing SUGT1 and α-tubulin expression (aI). aII is a magnification of the dashed region in aI. aII1–4 are the magnifications of the dashed regions in aII. Fluorescence overlap spectrums of aII1–4 cropped regions, are shown. b HIV-1 IN and SUGT1 expression levels by WB in control and SUGT1-depleted U2OS cells after 48 h siRNA transfection and expression of exogenous HIV-1 IN for 24 h. Representative confocal micrographs of HIV-1 HA-IN expression in control and SUGT1-depleted U2OS cells (c) and percentages of cells showing nuclear or diffused HIV-1 IN (d). e Immunoprecipitation of HA-IN in control and HA-IN-overexpressing HEK293T cells and expression of indicated proteins by WB. WB and images are representative of three independent experiments. Means ± SEM are indicated from at least three independent experiments. P values were calculated using the two-way ANOVA test (****p < 0.0001).
N Sim Module, supplied by Nikon, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nikon-structured+illumination+microscopy+(3d+n-sim)/Maximum+Magnification+Module/pmc03513318-205-15-19
Average 95 stars, based on 1 article reviews
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3d images  (Oxford Instruments)
99
Oxford Instruments 3d images
a Representative <t>SIM</t> micrograph of 4 h HIV-1ΔEnvNL4-3-GFP-Vpr <t>(VSV-G)-infected</t> <t>U2OS</t> cells showing SUGT1 and α-tubulin expression (aI). aII is a magnification of the dashed region in aI. aII1–4 are the magnifications of the dashed regions in aII. Fluorescence overlap spectrums of aII1–4 cropped regions, are shown. b HIV-1 IN and SUGT1 expression levels by WB in control and SUGT1-depleted U2OS cells after 48 h siRNA transfection and expression of exogenous HIV-1 IN for 24 h. Representative confocal micrographs of HIV-1 HA-IN expression in control and SUGT1-depleted U2OS cells (c) and percentages of cells showing nuclear or diffused HIV-1 IN (d). e Immunoprecipitation of HA-IN in control and HA-IN-overexpressing HEK293T cells and expression of indicated proteins by WB. WB and images are representative of three independent experiments. Means ± SEM are indicated from at least three independent experiments. P values were calculated using the two-way ANOVA test (****p < 0.0001).
3d Images, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nikon-structured+illumination+microscopy+(3d+n-sim)/Imaris/10__7554_slash_elife__33183-277-13-17
Average 99 stars, based on 1 article reviews
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illumination microscope  (Nikon)
93
Nikon illumination microscope
a Representative <t>SIM</t> micrograph of 4 h HIV-1ΔEnvNL4-3-GFP-Vpr <t>(VSV-G)-infected</t> <t>U2OS</t> cells showing SUGT1 and α-tubulin expression (aI). aII is a magnification of the dashed region in aI. aII1–4 are the magnifications of the dashed regions in aII. Fluorescence overlap spectrums of aII1–4 cropped regions, are shown. b HIV-1 IN and SUGT1 expression levels by WB in control and SUGT1-depleted U2OS cells after 48 h siRNA transfection and expression of exogenous HIV-1 IN for 24 h. Representative confocal micrographs of HIV-1 HA-IN expression in control and SUGT1-depleted U2OS cells (c) and percentages of cells showing nuclear or diffused HIV-1 IN (d). e Immunoprecipitation of HA-IN in control and HA-IN-overexpressing HEK293T cells and expression of indicated proteins by WB. WB and images are representative of three independent experiments. Means ± SEM are indicated from at least three independent experiments. P values were calculated using the two-way ANOVA test (****p < 0.0001).
Illumination Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nikon-structured+illumination+microscopy+(3d+n-sim)/Ti2-LAPP+Modular+Illumination+System/pm37459547-266-10-13
Average 93 stars, based on 1 article reviews
illumination microscope - by Bioz Stars, 2026-10
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orcaflash4 scmos camera  (Hamamatsu)
90
Hamamatsu orcaflash4 scmos camera
a Representative <t>SIM</t> micrograph of 4 h HIV-1ΔEnvNL4-3-GFP-Vpr <t>(VSV-G)-infected</t> <t>U2OS</t> cells showing SUGT1 and α-tubulin expression (aI). aII is a magnification of the dashed region in aI. aII1–4 are the magnifications of the dashed regions in aII. Fluorescence overlap spectrums of aII1–4 cropped regions, are shown. b HIV-1 IN and SUGT1 expression levels by WB in control and SUGT1-depleted U2OS cells after 48 h siRNA transfection and expression of exogenous HIV-1 IN for 24 h. Representative confocal micrographs of HIV-1 HA-IN expression in control and SUGT1-depleted U2OS cells (c) and percentages of cells showing nuclear or diffused HIV-1 IN (d). e Immunoprecipitation of HA-IN in control and HA-IN-overexpressing HEK293T cells and expression of indicated proteins by WB. WB and images are representative of three independent experiments. Means ± SEM are indicated from at least three independent experiments. P values were calculated using the two-way ANOVA test (****p < 0.0001).
Orcaflash4 Scmos Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nikon-structured+illumination+microscopy+(3d+n-sim)/orca+flash+4+0+camera/bio_rxiv__2020__03__06__981076-201-27-26
Average 90 stars, based on 1 article reviews
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nikon eclipse ti2 e  (Nikon)
99
Nikon nikon eclipse ti2 e
a Representative <t>SIM</t> micrograph of 4 h HIV-1ΔEnvNL4-3-GFP-Vpr <t>(VSV-G)-infected</t> <t>U2OS</t> cells showing SUGT1 and α-tubulin expression (aI). aII is a magnification of the dashed region in aI. aII1–4 are the magnifications of the dashed regions in aII. Fluorescence overlap spectrums of aII1–4 cropped regions, are shown. b HIV-1 IN and SUGT1 expression levels by WB in control and SUGT1-depleted U2OS cells after 48 h siRNA transfection and expression of exogenous HIV-1 IN for 24 h. Representative confocal micrographs of HIV-1 HA-IN expression in control and SUGT1-depleted U2OS cells (c) and percentages of cells showing nuclear or diffused HIV-1 IN (d). e Immunoprecipitation of HA-IN in control and HA-IN-overexpressing HEK293T cells and expression of indicated proteins by WB. WB and images are representative of three independent experiments. Means ± SEM are indicated from at least three independent experiments. P values were calculated using the two-way ANOVA test (****p < 0.0001).
Nikon Eclipse Ti2 E, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nikon-structured+illumination+microscopy+(3d+n-sim)/ECLIPSE+Ti2/pm39640577-227-31-31
Average 99 stars, based on 1 article reviews
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lu n3 sim laser  (Nikon)
93
Nikon lu n3 sim laser
a Representative <t>SIM</t> micrograph of 4 h HIV-1ΔEnvNL4-3-GFP-Vpr <t>(VSV-G)-infected</t> <t>U2OS</t> cells showing SUGT1 and α-tubulin expression (aI). aII is a magnification of the dashed region in aI. aII1–4 are the magnifications of the dashed regions in aII. Fluorescence overlap spectrums of aII1–4 cropped regions, are shown. b HIV-1 IN and SUGT1 expression levels by WB in control and SUGT1-depleted U2OS cells after 48 h siRNA transfection and expression of exogenous HIV-1 IN for 24 h. Representative confocal micrographs of HIV-1 HA-IN expression in control and SUGT1-depleted U2OS cells (c) and percentages of cells showing nuclear or diffused HIV-1 IN (d). e Immunoprecipitation of HA-IN in control and HA-IN-overexpressing HEK293T cells and expression of indicated proteins by WB. WB and images are representative of three independent experiments. Means ± SEM are indicated from at least three independent experiments. P values were calculated using the two-way ANOVA test (****p < 0.0001).
Lu N3 Sim Laser, supplied by Nikon, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nikon-structured+illumination+microscopy+(3d+n-sim)/LU-N4%2FN4S+4-laser+Unit%2C+LU-N3+3-laser+Unit/pm37256900-309-85-88
Average 93 stars, based on 1 article reviews
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axio observer 7 inverted widefield microscope  (Carl Zeiss)
98
Carl Zeiss axio observer 7 inverted widefield microscope
a Representative <t>SIM</t> micrograph of 4 h HIV-1ΔEnvNL4-3-GFP-Vpr <t>(VSV-G)-infected</t> <t>U2OS</t> cells showing SUGT1 and α-tubulin expression (aI). aII is a magnification of the dashed region in aI. aII1–4 are the magnifications of the dashed regions in aII. Fluorescence overlap spectrums of aII1–4 cropped regions, are shown. b HIV-1 IN and SUGT1 expression levels by WB in control and SUGT1-depleted U2OS cells after 48 h siRNA transfection and expression of exogenous HIV-1 IN for 24 h. Representative confocal micrographs of HIV-1 HA-IN expression in control and SUGT1-depleted U2OS cells (c) and percentages of cells showing nuclear or diffused HIV-1 IN (d). e Immunoprecipitation of HA-IN in control and HA-IN-overexpressing HEK293T cells and expression of indicated proteins by WB. WB and images are representative of three independent experiments. Means ± SEM are indicated from at least three independent experiments. P values were calculated using the two-way ANOVA test (****p < 0.0001).
Axio Observer 7 Inverted Widefield Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nikon-structured+illumination+microscopy+(3d+n-sim)/Inverted+microscope+Axio+Observer+7/bio_rxiv__2024__01__24__577101-339-34-33
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Image Search Results


a Representative SIM micrograph of 4 h HIV-1ΔEnvNL4-3-GFP-Vpr (VSV-G)-infected U2OS cells showing SUGT1 and α-tubulin expression (aI). aII is a magnification of the dashed region in aI. aII1–4 are the magnifications of the dashed regions in aII. Fluorescence overlap spectrums of aII1–4 cropped regions, are shown. b HIV-1 IN and SUGT1 expression levels by WB in control and SUGT1-depleted U2OS cells after 48 h siRNA transfection and expression of exogenous HIV-1 IN for 24 h. Representative confocal micrographs of HIV-1 HA-IN expression in control and SUGT1-depleted U2OS cells (c) and percentages of cells showing nuclear or diffused HIV-1 IN (d). e Immunoprecipitation of HA-IN in control and HA-IN-overexpressing HEK293T cells and expression of indicated proteins by WB. WB and images are representative of three independent experiments. Means ± SEM are indicated from at least three independent experiments. P values were calculated using the two-way ANOVA test (****p < 0.0001).

Journal: Cell Death and Differentiation

Article Title: SUGT1 controls susceptibility to HIV-1 infection by stabilizing microtubule plus-ends

doi: 10.1038/s41418-020-0573-5

Figure Lengend Snippet: a Representative SIM micrograph of 4 h HIV-1ΔEnvNL4-3-GFP-Vpr (VSV-G)-infected U2OS cells showing SUGT1 and α-tubulin expression (aI). aII is a magnification of the dashed region in aI. aII1–4 are the magnifications of the dashed regions in aII. Fluorescence overlap spectrums of aII1–4 cropped regions, are shown. b HIV-1 IN and SUGT1 expression levels by WB in control and SUGT1-depleted U2OS cells after 48 h siRNA transfection and expression of exogenous HIV-1 IN for 24 h. Representative confocal micrographs of HIV-1 HA-IN expression in control and SUGT1-depleted U2OS cells (c) and percentages of cells showing nuclear or diffused HIV-1 IN (d). e Immunoprecipitation of HA-IN in control and HA-IN-overexpressing HEK293T cells and expression of indicated proteins by WB. WB and images are representative of three independent experiments. Means ± SEM are indicated from at least three independent experiments. P values were calculated using the two-way ANOVA test (****p < 0.0001).

Article Snippet: For U2OS cells, acquisitions were performed in 3D SIM mode, with a N-SIM structured illumination Nikon microscope before image reconstruction using the NIS Elements software.

Techniques: Infection, Expressing, Fluorescence, Transfection, Immunoprecipitation